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Project Proposal Request for FY 2007 - FY 2009 Funding (Revised Summer 2006)

Proposal 200734400: Lower Columbia River Wild Coho DNA Stock Identification Proposal

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Table of Contents
Part 1. Administration and Budgeting
Section 1: General Administrative
Section 2: Project Location
Section 3: Project Species
Section 4: Past Accomplishments
Section 5: Relationship to Other Projects
Section 6: Biological Objectives
Section 7: Work Elements
Section 8: Budget
Section 9: Project Future
Section 10: Documents
Part 2. Reviews
Part 1 of 2. Administration and Budgeting
Section 1: General Administrative Information
Process Information:
Date Proposal Submitted & Finalized Status Form Generator
July 14, 2006 Finalized Bruce Crawford
Proposal Type: New
Proposal Number: 200734400
Proposal Name: Lower Columbia River Wild Coho DNA Stock Identification Proposal
Agency, Institution or Organization: Fish Friendly Inc.
Short Description: Fish Friendly Incorporated (FFI) in cooperation with the Washington Department of Fish and Wildlife (WDFW) proposes to develop a DNA baseline for naturally produced coho salmon in the Lower Columbia River tributaries.
Information Transfer: ·We propose to provide all data to the BPA Pisces system as it develops the capacity to receive it and to store it. ·Also, we propose to store information in the WDFW genetics database where it can be accessed for future Columbia River. reports. ·The data dictionary will be consistent with any Pacific Coastal Salmon Recovery Fund (PCSRF) reporting metrics provided to Congress ·All sites will be geo-spatially referenced and available on the Washington natural resourcedata portal at www.swim.wa.gov;
 
Project Proposal Contacts
Contact Organization Address Phone/Email Roles Notes
Form Submitter
Bruce Crawford Fish Friendly Inc. PO Box 14635
Tumwater, WA 98511		 Ph: 360-561-2302
Fax: [left blank]
Email: crawfordb@thurston.com		 Form Submitter
All Assigned Contacts
Bruce Crawford Fish Friendly Inc. PO Box 14635
Tumwater, WA 98511		 Ph: 360-561-2302
Fax: [left blank]
Email: crawfordb@thurston.com		 Contract Manager
Project Lead
James Shaklee [organization left blank] PO Box 360
Laporte CO 80535		 Ph: 970-484-8002
Fax: [fax left blank]
Email: jbsgenetics@direcway.com		 Technical Contact
Greg Volkhardt Washington Dept Fish and Wildlife 600 Capitol Way N
Olympia, WA 98501-1091		 Ph: (360) 902-2779
Fax: (360) 902-2944
Email: volkhgcv@dfw.wa.gov		 Interested Party
Section 2: Project Location
Sponsor Province: Lower Columbia ARG Province: No Change
Sponsor Subbasin: None Selected ARG Subbasin: No Change
Location(s) at which the action will be implemented
Latitude Longitude Waterbody Location Description County/State Subbasin Primary?
Abernathy Creek Wahkiakum, Washington Columbia Estuary No
Coweeman Cowlitz, Washington Cowlitz No
Cowlitz Cowlitz, Washington Cowlitz No
EF Lewis , Washington Lewis No
Elochoman Wahkiakum, Washington Elochoman No
Germany Wahkiakum, Washington Columbia Estuary No
Grays Wahkiakum, Washington Grays No
Hamilton Creek Skamania, Washington Columbia Gorge No
Kalama Cowlitz, Washington Kalama No
Mill Creek Wahkiakum, Washington Columbia Estuary No
NF Lewis , Washington Lewis No
NF Toutle Cowliz, Washington Cowlitz No
Rock Creek Clark, Washington Columbia Lower No
SF Toutle Cowlitz, Washington Cowlitz No
Skamokowa Wahkiakum, Washington Columbia Estuary No
Washougal , Washington Washougal No
WInd River , Washington Wind No
Section 3: Focal Species
Focal Species:
Primary Secondary Additional Species
Coho Lower Columbia River ESU
Section 4: Past Accomplishments
Past Accomplishments for Each Fiscal Year of This Project This proposal is for funding a new project, and has no past accomplishments.
Section 5: Relationships to Other Projects
Other Current Projects Related to this Project (any funding source)
Funding Source Related ID Related Project Title Relationship
PCSRF - WSRFB [no entry] Intensively monitored watersheds Juvenile sampling of presmolt and smolts is occurring in Abernathy, Mill, and Germany Creeks. Samples of DNA will be coordinated
PCSRF - WSRFB [no entry] SMolt Monitoring Juvenile migrants are sampled at Cedar Creek, NF Lewis. Coordination of DN samples will be made.
Other: NOAA [no entry] Kalama River Research WDFW Kalama team may be able to assist in obtaining DNA samples of juvenile coho.
BPA 198201301 Coded Wire Tag - PSMFC Data obtained will help calibrate CWT information about lower Columbia wild coho
BPA 200306300 Repro Success Abernathy Creek Some collection activities associtated with steelhead may be possible since activity will occur in the same stream
Section 6: Biological Objectives
Biological Objectives of this Proposed Project
Biological Objective Full Description Associated Subbasin Plan Strategy Page Nos
Measure baseline genetic diversity of coho 1. In order to be able to determine the phenotype of Lower Columbia River tributary coho populations, we intend to randomly select locations to sample within each stream in the ESU for a total of fifty sites within the ESU. Lower Columbia [7.6.4] Harvest 7-26]
Sample DNA of wild salmon in the fisheries we propose to place samplers upon cooperating boats in the charter fleet between Cape Falcon and Ledbetter Point and to enlist the assistance of the sport fishing community to help take samples of DNA in the Buoy 10 sport fishery. Lower Columbia 7.6.4 Harvest 7-26
Section 7: Work Elements
Work Elements and Associated Biological Objectives
Work Element Name Work Element Title Description Start Date End Date Estimated Budget
Collect/Generate/Validate Field and Lab Data Sample wild coho DNA in LCR tributaries Randomly select locations to sample within each stream in the ESU for a total of fifty sites within the ESU. Collect a minimum of 50 samples of DNA from every major tributary stream within the Lower Columbia River Coho ESU. 7/1/2007 9/30/2009 $323,375
Biological Objectives Metrics
Measure baseline genetic diversity of coho
 No Metrics for this Work Element
Collect/Generate/Validate Field and Lab Data Sample wild coho in Lower Columbia fisheries In order to be able to sample the sport fishery for coho, we propose to place samplers upon cooperating boats in the charter fleet between Cape Falcon and Ledbetter Point and to enlist the assistance of the sport fishing community to help take samples of DNA in the Buoy 10 sport fishery. 7/1/2009 10/31/2009 $76,057
Biological Objectives Metrics
Sample DNA of wild salmon in the fisheries
 Secondary R, M, and E Type: DNA array
Secondary R, M, and E Type: Location
Section 8: Budget
Itemized Estimated Budget
Item Note FY 2007 Cost FY 2008 Cost FY 2009 Cost
Personnel [blank] $56,771 $56,771 $97,542
Fringe Benefits [blank] $11,354 $11,354 $27,140
Travel [blank] $3,500 $3,500 $7,000
Supplies [blank] $1,000 $1,000 $2,000
Other Laboratory Work $31,500 $31,500 $45,500
Other Publications/permits $500 $500 $2,000
Other Equipment $7,000 $1,000 $1,000
Totals $111,625 $105,625 $182,182
Total Estimated FY 2007-2009 Budgets
Total Itemized Budget$399,432
Total Work Element budget$399,432
Cost sharing
Funding Source or Organization Item or Service Provided FY 2007 Est Value ($) FY 2008 Est Value ($) FY 2009 Est Value ($) Cash or in-kind? Status
Section 9: Project Future
Project Future Costs and/or Termination
FY 2010 Est Budget FY 2011 Est Budget Comments
$76,057 $76,057 Allows completion of sampling of the fishery
Future Operations & Maintenance Costs
None
 
Termination Date Comments
12/31/2011 Provides time for analysis and report completions
 
Final Deliverables
Coho DNA baseline profiles for Washington Lower Columbia River tributaries. DNA profile of sport caught wild coho and commercial caught wild coho All data Final Report
Section 10: Narrative
Document Type Size Date
Fix-it Loop Documents
Documents Originally Submitted with this Proposal
Narrative for proposal 200734400 doc 198 kb 1/10/2006
Part 2 of 2. Reviews of Proposal
Administrative Review Group (ARG) Results
Account Type:
Expense		 No changes were made to this proposal


BPA's in lieu Funding Review of new project proposals (August 3, 2006) [Download letter and table]
BPA's in lieu Rating: 3.0
Approx. BPA share of total costs: BPA 100%
Status of Cost Share:
Notes: DNA research wild coho, fishery managers authorized/required


NPCC Final Funding Recommendations (October 23, 2006) [Full NPCC Council Recs]
FY 2007 NPCC Rec
$ 0		 FY 2008 NPCC Rec
$ 0		 FY 2009 NPCC Rec
$ 0		 Total NPCC Rec
$ 0
Budget Type:Expense
Budget Category:ProvinceExpense
Recommendation:Do Not Fund
NPCC Comments:


NPCC Draft Funding Recommendations (September 15, 2006) [Full NPCC Council Recs]
FY 2007 NPCC Rec
$ 0		 FY 2008 NPCC Rec
$ 0		 FY 2009 NPCC Rec
$ 0		 Total NPCC Rec
$ 0
FY 2007 MSRT Rec
$ 0		 FY 2008 MSRT Rec
$ 0		 FY 2009 MSRT Rec
$ 0		 Total MSRT Rec
$ 0
Budget Category:ProvinceExpense
NPCC Comments:

Local or MSRT Comments: See Washington guidance


Independent Scientific Review Panel Final Review (August 31, 2006) [Download full document]
Recommendation: Not fundable
Comments: The sponsors responded to the questions raised about this project in the preliminary review. The sponsor’s response did not materially change the impression the ISRP has about this effort. The sponsor was asked to summarize the status of tissue collections and DNA genotyping of coho salmon. It was hoped that this would identify a deficiency in data from wild Columbia River coho, in contrast to other collections. The sponsor summarized limited published literature on genotyping coho salmon; it does not appear to be exhaustive, or recent. Most of the citations are from the early to mid-1990s. The sponsors were requested to provide evidence that Washington Department of Fish and Wildlife (WDFW) was a cooperator. They provided a letter indicating that WDFW would be willing to serve as the contractor of the genotyping if space and time is available in the laboratory. WDFW did not express particular interest in the work, and no information is provided on the genes that will be analyzed. Finally, the sponsors were asked to provide evidence of the need for the data. The response was not convincing. The coho status review that was cited was from 1995, not the more recent update, and there is no link to the Lower Columbia Salmon Recovery Plan, Lower Columbia Technical Recovery Team, or harvest management organization (Pacific Salmon Commission or Pacific Fishery Management Council) indicating that there is an urgent need for this data. In conclusion, the ISRP is unable to justify the need to collect this data, on the basis of the proposal.


Independent Scientific Review Panel Preliminary Review (June 2, 2006) [Download full document]
Recommendation: Not fundable
Comments: Although additional information on coho genetics is worthwhile, the proposal provides little or no discussion of current status of coho DNA collections and analyses of existing data and other ongoing efforts. The proposal indicates that WDFW is a cooperator, but no indication is given that they are interested in doing the work or are on board. No use or demand for this data is explicitly identified in the proposal. Integration with existing genetics efforts is not articulated.

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