| Work Element Name |
Work Element Title |
Description |
Start Date |
End Date |
Estimated Budget |
| 01: Coordination |
Duncan Creek project planning and coordination |
Duncan Creek project planning and coordination. This includes things such as annually updating broodstock collection curves and goals, population estimation marking protocols, annual work plans, scheduling, attending coordination meetings with other researchers conducting work in the same areas at the same times of the year. |
10/1/2006 |
9/30/2009 |
$17,500 |
| Biological Objectives |
Metrics |
|
No Metrics for this Work Element |
|
| 02: Manage and Administer Projects |
Duncan Creek project management and administration |
Complete all BPA, PSMFC and WDFW administrative requirements. |
10/1/2006 |
9/30/2009 |
$17,500 |
| Biological Objectives |
Metrics |
|
No Metrics for this Work Element |
|
| 03: Produce Environmental Compliance Documentation |
NOAA 4d permit compliance |
Annually apply for renewal of NOAA 4d permit. Renewal requires an annual report of take associated with current permit be submitted to NOAA via an online form. |
10/31/2006 |
1/31/2009 |
$3,200 |
| Biological Objectives |
Metrics |
|
No Metrics for this Work Element |
|
| 04: Install Fish Monitoring Equipment |
Install/remove adult trap |
Annually install and remove adult trap at mouth of Duncan Creek. |
11/1/2006 |
1/15/2009 |
$9,500 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|
| 05: Collect/Generate/Validate Field and Lab Data |
Evaluate natural re-colonization of Duncan Creek by chum salmon, adult trap monitoring |
Check trap daily for fish presence. Enumerate, collect and record biological information (sex, fork length, marks/clips, condition, collect scales for aging) on all salmonid adults. Tag (double Floy tag) and release all adult salmonids, except chum salmon, above the trap in Duncan Creek. All chum salmon recovered in the trap will be sampled, Floy tagged and released into the spawning channels. Trap will be operated annually during periods of adult chum salmon presence, November 1 through January 15. |
11/1/2006 |
1/15/2009 |
$26,500 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Primary R, M, and E Type: Action Effectiveness Research Focal Area: Tributaries
|
|
| 06: Collect/Generate/Validate Field and Lab Data |
Monitor physical characteristics of renovated spawning channels |
Temperature loggers will be employed to record intra-gravel and surface water temperatures, mini-piezometers will be placed in the spawning areas to measure vertical hydraulic gradients (VHG) and intra-gravel dissolved oxygen (DO) levels. A digital flow meter will be used to document water velocities in the spawning areas. These activities will take place annually from November 1 to May 30. In addition, gravel samples will be removed and analyzed annually to document the gravel composition in the spawning channels to track any significant invasion of fines into these areas. |
11/1/2006 |
8/30/2009 |
$18,900 |
| Biological Objectives |
Metrics |
Habitat Status Monitoring
|
Focal Area: Tributaries Primary R, M, and E Type: Action Effectiveness Research
|
|
| 07: Trap/Collect/Hold/Transport Fish - Hatchery |
Seining activities for adult broodstock collection |
Seine mainstem Columbia River spawning locations to collect broodstock for adult supplementation into the spawning channels and for artificial propagation at Washougal Hatchery. Mainstem spawning locations suitable for broodstock collection will be seined using a beach seine set from a boat. All adults captured will be enumerated and examined for suitability to be used as broodstock. Female suitable for use in the hatchery program will show no signs of spawning/redd digging activities; they will have a "soft" full/round abdomen and when examined and the belly area around the ovidepositor will not "suck" in noticeably. Females suitable for supplementation at the channels will show no signs of spawning/redd digging activity; they will have a full/round abdomen (green). Male adults need to produce normal looking milt when checked in the field to be suitable for the hatchery or channels. This activity will occur annually beginning in early November and ending in early to mid-January. |
11/1/2006 |
1/15/2009 |
$268,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
* Purpose of production program: Supplementation * # of non-clip (natural origin) adults: 1,200 - 6,000 * Purpose of production program: Research
|
|
| 08: Trap/Collect/Hold/Transport Fish - Hatchery |
Transport supplementation adults |
Transport supplementation adults to Duncan Creek spawning channels. |
11/1/2006 |
1/15/2009 |
$3,200 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
* Purpose of production program: Supplementation * # of non-clip (natural origin) adults: 120 - 400
|
|
| 09: Produce Hatchery Fish |
Produce chum salmon fed-fry |
Transport, spawn, incubate, rear and release all chum salmon fry needed for supplementation releases at Duncan Creek and any “salvage” plan implementation.
|
11/1/2006 |
5/31/2009 |
$66,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
* Purpose of production program: Supplementation * Purpose of production program: Research * Broodstock collection: # of non-clip (natural origin) fish: 60-400 * Production: # smolts released from program: 85,000 - 490,000
|
|
| 10: Collect/Generate/Validate Field and Lab Data |
Sampling of adults used for artificial propagation |
Collect biological data on adults used for artificial propagation at Washougal Hatchery. Biological data needed for formulation of predictive regression formulas used to estimate fecundity of females that spawned naturally in the spawning channels. Tissue samples for DNA analysis and otoliths will also be recovered |
11/1/2006 |
1/15/2009 |
$12,300 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Secondary R, M, and E Type: Status and Trend Monitoring Focal Area: Hydrosystem Primary R, M, and E Type: Action Effectiveness Research
|
|
| 11: Collect/Generate/Validate Field and Lab Data |
Sampling of spawners placed into spawning channels |
Collect biological data on adults placed into spawning channels. Egg retention values will be obtained from each female. At death, each adults lengths (fork and mid-eye-to-hypural plate) will be recorded, scale, tissue samples for DNA analysis and otoliths will be collected. |
11/1/2006 |
1/15/2009 |
$12,300 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Primary R, M, and E Type: Action Effectiveness Research Secondary R, M, and E Type: Status and Trend Monitoring Focal Area: Tributaries
|
|
| 12: Collect/Generate/Validate Field and Lab Data |
Adult sampling and marking for spawning population estimation |
Collect and record biological data on adults seined but not used for supplementation and mark adults for population estimation. Data collected includes, but is not limited to, sex, fork length, tags/marks, scales, DNA tissue samples, condition and spawn condition for females. Place unique primary and batch secondary tags/marks on all adults seined but not used for supplementation prior to release. Recaptures will have the tag number and their condition recorded prior to release. Conduct a carcass tagging program on Hamilton Creek. Data collected includes, but is not limited to, sex, fork length, tags/marks, scales, DNA tissue samples, condition and spawn success for females. Place two unique primary tags on all chum salmon adults recovered and release for redistribution. Recaptures will have tag numbers recorded then the carcass will be marked to prevent multiple tagging/sampling. |
11/1/2006 |
1/15/2009 |
$268,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Primary R, M, and E Type: Status and Trend Monitoring Focal Area: Hydrosystem Focal Area: Tributaries Secondary R, M, and E Type: Action Effectiveness Research
|
|
| 13: Collect/Generate/Validate Field and Lab Data |
Sampling of eggs for individual female fecundity |
Sampling to determine fecundity of females spawned at Washougal Hatchery. For each female individually spawned and incubated, when those eggs are shocked and picked, the number of dead eggs and a total weight for all viable eyed-eggs will be recorded. Five sub-samples of viable eyed-eggs will be weighed and hand counted to calculate estimates of the total number of eyed eggs for each female. These estimates will be used to calculate a mean number of eyed eggs, with 95% CI. The mean number of eyed eggs plus the number of non-viable eggs removed equals that female’s fecundity. This information, along with phenotypic data collected at spawning, completes the data needed for formulation of predictive regression formulas for fecundity estimates of females that spawned in the spawning channels. |
1/1/2007 |
2/15/2009 |
$6,100 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Primary R, M, and E Type: Action Effectiveness Research
|
|
| 14: Mark/Tag Animals |
Thermally mark embryonic chum salmon fry |
Thermally mark embryonic chum salmon at Washougal Hatchery. Two (a pre- and post-hatching) bar codes (thermal marks) will be placed to identify the fry's origin and release location. Thermal marks are created by manipulating temperatures during the stages between eyed and yolk absorption. Each time the water temperature is dropped by 2-4 degrees C a distinctive black band is deposited in the microstructure of a developing otolith. Exposure to chilled water for periods of 8 to 48 hours will essentially create bar codes on the otoliths that can be read. The bar codes will be determined by personnel in the WDFW's Otolith Lab. The Otolith Lab will provided a schedule for chilled water applications. Each years thermal mark will be unique. Unique thermal marks will also be used to identify releases at areas other than Duncan Creek if they occur. |
1/1/2007 |
2/15/2009 |
$6,250 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Hydrosystem Focal Area: Tributraries Primary R, M, and E Type: Action Effectiveness Research
|
|
| 15: Collect/Generate/Validate Field and Lab Data |
Sampling of chum fry and collection of thermally marked voucher fry |
Each Heath incubation tray will need to be sampled prior to ponding to ensure the fry are ready to be ponded. If the Heath tray contains the fry from an individually spawned and incubated female, it needs to be sampled at ponding to determine egg-to-fry survival rates. Trays that contain fry from multiple females will be sampled only for mortalities and abnormalities. Voucher fry from each thermally marked group will be collected to check for mark presence, validation and clarity. |
3/15/2007 |
4/30/2009 |
$16,000 |
| Biological Objectives |
Metrics |
Population/Biological Status Monitoring
|
Primary R, M, and E Type: Action Effectiveness Research
|
|
| 16: Rear Fish |
Rear chum salmon fry |
Rearing of strontium marked voucher fry during the “growing-out” period needed for mark analysis. |
2/15/2007 |
5/31/2009 |
$1,600 |
| Biological Objectives |
Metrics |
Population/Biological Status Monitoring
|
* Purpose of production program: Research # of smolts into program: 60
|
|
| 17: Analyze/Interpret Data |
Analysis of hatchery data |
Calculate green and eyed-egg stage fecundity estimates for each individual female chum salmon spawned. Calculate green-egg to eyed-egg and green-egg to fry survival rates for each individually spawned female, and an overall green-egg to release survival rate. Mean weights, lengths, K values and coefficient of variation statistics for each of these parameters and frequency distributions of lengths and weights for fry at release. These estimates and survival rates will need to be generated each brood year. |
11/1/2006 |
6/30/2009 |
$16,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring
|
Primary R, M, and E Type: Action Effectiveness Research
|
|
| 18: Install Fish Monitoring Equipment |
Install fry traps at monitoring weirs |
Annually install and remove fence panel weirs and live boxes at spawning channel monitoring weirs. |
2/10/2007 |
5/31/2009 |
$3,200 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|
| 19: Collect/Generate/Validate Field and Lab Data |
Enumeration and sampling of naturally produced fry |
Collect and record biological data from out-migrating salmonid fry caught in fry traps installed on the two monitoring weirs at the spawning channels. |
2/10/2007 |
5/15/2009 |
$82,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Tributaries Primary R, M, and E Type: Action Effectiveness Research
|
|
| 20: Mark/Tag Animals |
Strontium mark naturally produced fry |
Place strontium mark on naturally produced fry via six hour immersion in a 2000ppm bath of strontium chloride hexa-6-hydrate. Remove, decode and archive voucher fry otoliths |
2/10/2007 |
8/31/2009 |
$16,000 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Tributaries Primary R, M, and E Type: Action Effectiveness Research Focal Area: Hydrosystem
|
|
| 21: Analyze/Interpret Data |
Estimate egg-to-fry survival rates in spawning channels |
Predictive regression formulas will be developed using phenotypic measurements and fecundity data collected from females spawned at Washougal Hatchery. These formulas will be used in conjunction with phenotypic data collected from females placed in the spawning channels to generate a potential egg deposition (PED) value, with 95% CI, for each individual female that spawned in the channels. Each female was sampled soon after death (within 24 hours) to document any retained eggs. This number of retained eggs is then subtracted from the females PED to generate an actual egg deposition (AED) value, with 95% CI. Egg-to-fry survival rates, with 95% CI, are calculated by dividing the number fry trapped by the summed AED values for all females that spawned in each channel. |
5/1/2007 |
6/30/2009 |
$16,500 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Tributaries Primary R, M, and E Type: Action Effectiveness Research
|
|
| 22: Analyze/Interpret Data |
Analysis of adult sampling and marking for population estimates |
Population estimates, with 95% CI, will be calculated annually for each seining location using the live mark/recapture data that will be generated in conjunction with seining for broodstock collection. Statistical test (Kolmogorov-Smirnov, chi-square test and Goodness of Fit test) will be used to test assumptions. Jolly-Seber population software will be used to estimate spawning population size; Akiake Information Criteria will be used to select the most parsimonious model at each area sampled. A population estimate, with 95% CI, will be calculated annually for Hamilton Creek using carcass mark/recapture data. Statistical test (Kolmogorov-Smirnov, chi-square test and Goodness of Fit test) will be used to test assumptions. Jolly-Seber population software will be used to estimate spawning population size; Akiake Information Criteria will be used to select the most parsimonious model. Maximum Likelihood Equations will be used to determine apparent residence time. |
1/15/2007 |
7/31/2009 |
$17,250 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Hydrosystem Primary R, M, and E Type: Status and Trend Monitoring Focal Area: Tributaries Primary R, M, and E Type: Status and Trend Monitoring
|
|
| 23: Analyze/Interpret Data |
Analysis to determine project origin adult returns |
Using the population estimates generated by this, and other projects, and the results of otolith decoding, estimate the contribution of project origin adults to spawning populations. These estimates will be generated annually. |
6/1/2007 |
7/31/2009 |
$17,250 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Tributaries Focal Area: Hydrosystem Primary R, M, and E Type: Action Effectiveness Research Secondary R, M, and E Type: Status and Trend Monitoring
|
|
| 24: Collect/Generate/Validate Field and Lab Data |
Decode otoliths collected from fall 2006-08 spawners |
Process and decode otoliths for thermal and strontium marks |
3/15/2007 |
8/30/2009 |
$33,358 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
Focal Area: Hydrosystem Primary R, M, and E Type: Action Effectiveness Research Focal Area: Tributaries Primary R, M, and E Type: Action Effectiveness Research
|
|
| 25: Disseminate Raw/Summary Data and Results |
Ad hoc in-season reports |
Produce and distribute to interested parties short in-season memos on project activities |
1/15/2007 |
8/31/2009 |
$3,200 |
| Biological Objectives |
Metrics |
Habitat Status Monitoring Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|
| 26: Produce Annual Report |
Generate annual reports |
Generate annual reports for the Duncan Creek project covering activities in FY07, 08 and 09 |
6/1/2007 |
9/30/2009 |
$52,000 |
| Biological Objectives |
Metrics |
|
No Metrics for this Work Element |
|
| 27: Produce Status Report |
Pisces status reporting |
Complete Pisces status reporting |
10/1/2006 |
9/30/2009 |
$3,200 |
| Biological Objectives |
Metrics |
|
No Metrics for this Work Element |
|
| 28: Develop RM&E Methods and Designs |
Explore the use of carcass weirs |
Explore the feasibility of using carcass weirs at mainstem spawning locations to increase recovery rates of carcasses, install one carcass weir. Increasing carcass recovery rates will benefit both population estimation and recovery of otoliths for origin determination work elements |
10/1/2006 |
9/30/2008 |
$6,500 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|
| 29: Install Fish Monitoring Equipment |
Install/remove carcass weir |
Install/remove carcass weir at one additional mainstem spawning location to evaluate. |
10/15/2007 |
12/31/2007 |
$12,800 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|
| 30: Install Fish Monitoring Equipment |
Install/remove carcass weirs |
Implement the use of carcass weirs at all suitable mainstem spawning locations. |
10/15/2008 |
1/15/2009 |
$19,300 |
| Biological Objectives |
Metrics |
Hatchery Status Monitoring Population/Biological Status Monitoring
|
No Metrics for this Work Element |
|